FastQCFastQC Report
Mon 27 Feb 2023
SRR3127033.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127033.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2851878
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408731.4331959501773919No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42940.1505674506412967No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG28820.10105621628975713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT127000.085.996721
ATAGGGC18350.077.0897143
TAGGGCA16050.072.9105454
CGTAGGG8300.072.5172962
ATAGGGA24900.070.966683
AGTAGGG47600.070.534412
AGATAGG15650.070.3880841
GTAGGGC15950.070.126523
GAATAGG18600.069.3482061
GTAGGGA22550.068.7754443
AGGGATG41250.068.472555
TAGAGGG54400.067.5954742
GGTAAGG15500.067.1209641
ATAAGGG39950.066.738442
AGTAAGG15050.066.312741
AATAGGG43350.066.0599062
TAGGGCG5000.065.795174
AAGAGGG98100.065.717072
AGAGGGC39800.065.417313
TAGGGAC12650.065.386514
Produced by FastQC (version 0.11.5)