FastQCFastQC Report
Mon 27 Feb 2023
SRR3127031.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127031.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603905
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67231.1132545681853934No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG30810.5101795812255239No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG29460.4878250718242108No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC27380.45338256845033575No Hit
AGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT11140.1844660998004653No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT9300.15399773143126816No Hit
GGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT8500.14075061474900855No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT8260.13677647974433066No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT7990.13230557786406802No Hit
AGGCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT7400.12253582931090155No Hit
ACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG7340.12154229555973207No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT6690.11077901325539613No Hit
ACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT6210.10283074324604037No Hit
GAAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT6160.10200279845339912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAAGG450.094.248071
GTAGGGC2250.087.9210743
GCGTAGG700.087.516061
CGTTTTT24400.085.750291
TAGCGGG2000.084.781032
CGGGAAT1900.084.0968255
TAGTAGG1800.083.776061
CGGGAAC1300.083.14555
CGAGGGA2000.082.4259953
CGAAGGG4250.080.902152
TAAGCGG1350.080.285391
CGGGATA650.079.530485
AGGGTAT4650.078.8307955
AGGGAAT11800.077.661695
AGGGTCG800.076.3673255
AGGGCAT5850.076.317125
GACGGGT507.094059E-1175.360913
AGTAGGG7950.075.2424242
AGTAAGG3850.074.6640551
ATAGGGT1900.074.369333
Produced by FastQC (version 0.11.5)