FastQCFastQC Report
Mon 27 Feb 2023
SRR3127030.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127030.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640005
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71821.1221787329786488No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG31840.49749611331161475No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG29760.4649963672158811No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC27700.43280911867876026No Hit
AGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT10790.1685924328716182No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT10040.1568737744236373No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT8960.13999890625854486No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT8610.13453019898282045No Hit
GGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT8350.13046773072085374No Hit
ACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG7790.1217177990796947No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT6910.10796790650073047No Hit
AGGCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT6530.10203045288708681No Hit
GAAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT6440.10062421387332912No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG201.8377792E-593.994777
CGTTTTT23200.085.809861
ACGGGTA550.085.449784
CGAAGGG4350.085.4049452
CTAGCGG750.081.576841
AGTAGGG9150.078.634932
ACGTAGG600.078.439281
AGGGTAC1200.078.328975
GTAGGGT2050.077.946883
AAGGGAC5450.077.610364
CGGGAAC1400.077.2099845
GGAGCTA21150.077.106829
AGGGTAT4950.076.9048165
GGCCGAT551.8189894E-1276.904817
CGAGGGA2150.076.507373
TAGCGGG3150.076.138592
TAGTAGG2350.076.102791
GAGGGTA3350.075.756974
GATCGGA255.5590295E-575.195819
AGGGAAT10850.074.935925
Produced by FastQC (version 0.11.5)