SRR3127029.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3127029.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1031296
Sequences flagged as poor quality	0
Sequence length	100
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12749	1.236211524140499	No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	5311	0.5149830892391709	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG	3107	0.3012714099540772	TruSeq Adapter, Index 18 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG	2893	0.2805208204046171	RNA PCR Primer, Index 34 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	2606	0.25269175871912625	RNA PCR Primer, Index 34 (96% over 25bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC	1442	0.13982406602953953	No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT	1315	0.1275094638202805	RNA PCR Primer, Index 34 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT	1257	0.12188547226014644	RNA PCR Primer, Index 34 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT	1182	0.11461306938066278	RNA PCR Primer, Index 34 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	4440	87.58309	1
CGTAGGG	245	84.401985	2
ATAGCGG	215	83.10801	1
AGGGATG	3290	82.72026	5
GGTAAGG	680	79.52188	1
ATGCGGG	550	79.46691	2
CGCTACG	900	79.37966	3
TCCGCTA	920	77.68792	1
ACGACCA	900	77.29447	7
GCTACGA	930	76.82275	4
CGGGATG	485	76.56223	5
AGAGGGC	1345	76.5297	3
AGGGAAT	2170	76.46175	5
ATAGGGA	1480	76.218025	3
AAGGGTA	845	76.20669	4
GATGACC	1500	76.1455	8
CTACGAC	945	75.60334	5
GGATGGC	2370	74.57079	7
AGAGGGA	3060	74.495224	3
AGGGTAT	625	74.45338	5