FastQCFastQC Report
Mon 27 Feb 2023
SRR3127028.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127028.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824458
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93001.1280137981558793No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39340.47716196580056236No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG29660.3597514973473482TruSeq Adapter, Index 18 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG28730.34847135936578943RNA PCR Primer, Index 34 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC25810.3130541519398199RNA PCR Primer, Index 34 (96% over 25bp)
AAAGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT14250.17284082391098152RNA PCR Primer, Index 34 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT12830.15561738742301004RNA PCR Primer, Index 34 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT12560.15234250865417037RNA PCR Primer, Index 34 (95% over 23bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10430.12650735392221313No Hit
GGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT10000.12129180625332035RNA PCR Primer, Index 34 (95% over 23bp)
AGAGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT8820.10697937311542856RNA PCR Primer, Index 34 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2350.088.235142
CGTTTTT32800.085.3952261
AGGGATG24150.083.8694155
AAGGGTA6200.083.609914
CGCTACG7950.082.988463
AGGGTAC3850.081.781975
CGAAGGG7300.081.340052
GGTAAGG5450.081.3302841
ATAGCGG2050.080.507281
GCTACGA8250.079.970694
GGGTACG650.079.52856
AGGGCGA3200.079.3025745
GCGTAGG1550.079.097471
ACGGGTA600.078.542654
GGATGGC19000.077.91137
TCCGCTA8600.077.859371
TAGCGGG4000.077.757222
CTACGAC8550.077.499075
ACGACCA8300.077.002417
GGGTATC3800.076.6746146
Produced by FastQC (version 0.11.5)