FastQCFastQC Report
Mon 27 Feb 2023
SRR3127027.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127027.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838456
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95791.1424570877899376No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39590.47217743089679126No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG27250.3250021468031715TruSeq Adapter, Index 18 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG26900.32082780730294735RNA PCR Primer, Index 34 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC25630.30568091825927657RNA PCR Primer, Index 34 (96% over 25bp)
AAAGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT13360.15934050206570172RNA PCR Primer, Index 34 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT12240.14598261566498422RNA PCR Primer, Index 34 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT12190.14538628145066645RNA PCR Primer, Index 34 (95% over 23bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC11740.14001927352180674No Hit
GGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT10440.12451458394954537RNA PCR Primer, Index 34 (95% over 23bp)
AGAGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT9320.11115669754882786RNA PCR Primer, Index 34 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA409.094947E-1293.997747
CCATACG156.902217E-493.997749
CGTAGGG2350.088.0241242
ACGGGTA600.086.16464
CGTTTTT31100.085.470441
AAGGGTA6000.085.381284
AGGGATG27350.084.202715
GCTACGA7050.081.9980244
CGCTACG7050.081.3313753
GGATGGC20500.080.700497
ATAGCGG2100.080.6512451
TAGCGGG3700.080.0489652
TAGGGCA4950.079.7556464
AGGGTAT5550.078.754855
CTACGAC7350.078.651175
AAGGGCG4550.078.50364
GCGGGAT5350.078.185034
AGGGTAC3950.077.339915
TCCGCTA7550.077.268531
ACGACCA7500.076.4514857
Produced by FastQC (version 0.11.5)