FastQCFastQC Report
Mon 27 Feb 2023
SRR3127026.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127026.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930118
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT609192.079063027495821No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT42170.1439191186157008No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT39440.13460208769749205No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33840.1154902294037305No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32930.11238455243099425No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT29730.10146349054884478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT192350.088.555551
ACGGGAT9000.072.587814
ATAGGGC18650.072.3263
ATAGCGG6150.070.33191
AGGGATG50500.069.984695
GAATAGG18750.069.206591
AGTAGGG46850.068.417342
TAGAGGG53350.068.098332
TAAGGGA28450.067.897063
AGAGGGC48250.067.211433
TAGGGCA17300.066.55974
AATAGGG46700.065.9197852
AGTAAGG20200.065.8680341
AGGGAAT38250.065.366735
ATAGGGA28400.065.2032553
GAGGGAT37400.064.592624
AGGGCAT26650.064.544685
GATAGGG43850.064.523552
AAGAGGG107200.064.185612
TAGGGTA8150.064.0113144
Produced by FastQC (version 0.11.5)