FastQCFastQC Report
Mon 27 Feb 2023
SRR3127025.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127025.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312401
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471392.0385305143874266No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT44790.19369477871701318No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT40210.1738885253898437No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT29570.12787574473458538No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27120.11728069655738775No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24330.10521531516376269No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG23920.1034422662851296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT153200.088.6790541
ACGGGAT5150.077.788034
ATAGGGC16100.074.9402543
TAGGGCA14850.074.2660754
AGGGATG37550.072.587015
ATAGGGA21600.070.6955953
AGTAGGG35300.069.0296552
AGTAAGG14500.068.945261
TAGAGGG38850.068.787882
AGGGAAT26400.067.998665
ATAGCGG4800.067.786691
AATAGGG35150.067.446992
AGGGTAT11200.066.714465
GAATAGG15400.066.447031
TACGGGT1850.066.2373053
AAGGGTA15700.066.042594
AGATAGG13950.065.916931
AGAGGGT24900.065.868983
AGAGGGC33850.065.718013
GGTAAGG13050.065.404051
Produced by FastQC (version 0.11.5)