FastQCFastQC Report
Mon 27 Feb 2023
SRR3127024.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127024.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422856
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491182.027276899658915No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT43840.18094348157711396No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT41610.17173946780163576No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT29720.1226651522005435No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28630.11816632932374026No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27610.11395642167755739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT153000.087.386271
ATAGGGC14650.072.5022053
CGTAGGG7050.070.691552
AGTAGGG37150.068.59482
AGGGATG40850.068.109865
TAGGGCA14850.068.04444
ACGGGAT7400.067.956734
AGGGCAT20350.067.6680455
TAAGGGA22000.067.2927553
CGGGATG9150.067.286915
TAGAGGG41250.066.791992
ATAGGGA22700.066.4598853
GATAGGG33100.066.334522
GGTAAGG17100.066.326081
AGGGAAT28200.065.830695
ATGAGGG36800.065.7976152
AAGGGCG9250.065.543314
TATAGGG19050.065.156792
AAGGGTA17000.064.967974
AGAGGGC35650.064.334023
Produced by FastQC (version 0.11.5)