FastQCFastQC Report
Mon 27 Feb 2023
SRR3127023.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127023.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3201678
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324821.0145305055661438No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56780.17734450497520363No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40540.1266211030590834No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG37710.11778198807000578No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG34720.10844313513101568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103150.083.871971
CGTAGGG10900.073.302892
ATAGGGA30200.073.301453
ATAGCGG7800.072.938871
ACGGGAT7900.071.9897164
ATAGGGC21500.071.265253
AGTAGGG52600.071.214962
AGGGTAC12850.070.2258155
TAGGGTA11050.069.7579654
TATAGGG23050.069.3276752
TAGGGCA18450.069.037514
TAGAGGG55400.068.63372
TAAGGGA27850.068.348423
AAGAGGG111300.068.325372
ATAGAGG22300.068.102941
AGGGATG48750.067.969365
GGTAAGG19700.067.7829361
AGGGCAT30350.067.519075
ATGCGGG16500.067.2243652
AGTTAGG10500.067.1692051
Produced by FastQC (version 0.11.5)