FastQCFastQC Report
Mon 27 Feb 2023
SRR3127022.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127022.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505347
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239160.9545983051449559No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44040.17578403310998436No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG35900.14329352381127244No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29700.11854645284665158No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG28170.11243951436667257No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC27110.10820856352433417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75200.084.908741
CGATTAC600.078.323049
CGTAGGG8050.074.355982
AGGGTAC9850.072.518395
TAGGGCA13950.071.96374
ACGGGAT5800.071.509344
ATAGGGA23950.071.434693
AGGGCAT22250.071.388375
ATAGGGC16300.071.1304553
ATAGCGG5000.070.7363741
AGTAGGG40200.070.696732
TAGAGGG42650.070.3928452
TATAGGG18750.070.382522
AGTTAGG7500.069.793231
AGGGATG38150.069.720855
TAGCGGG11500.069.672142
GGTAAGG14250.068.833531
AGATAGG16050.068.165491
AAGAGGG82750.068.119472
TAAGGGA21350.067.9924853
Produced by FastQC (version 0.11.5)