FastQCFastQC Report
Mon 27 Feb 2023
SRR3127021.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127021.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2644004
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248980.9416778492014385No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48520.18350955596133742No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG34950.1321858817157614No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31190.11796502577151925No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG28490.10775324091794113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76250.083.8648451
TAGGGCA13900.074.380244
ACGGGAT5950.074.243874
CGTAGGG8950.073.018082
GGTAAGG17200.070.581611
ATAGCGG6650.070.050931
ATAGGGA25700.069.852183
AGTAGGG43200.069.652212
TAAGGGA24900.069.642883
ATAGGGC16100.069.470563
TAGGGTA8050.069.470564
AGGGTAC11100.069.433755
GTAGGGA20250.069.389843
AGTAAGG18000.069.013131
TAGCGGG12650.068.757472
TATAGGG19750.068.558932
AAGAGGG90250.068.035352
GTAGGGT10450.067.906353
AGGGCAT23400.067.8813555
GTAAGGG43500.066.794062
Produced by FastQC (version 0.11.5)