FastQCFastQC Report
Mon 27 Feb 2023
SRR3127020.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127020.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3662054
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330670.9029632004334179No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT71880.19628328801268358No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG55600.15182736245833622No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG49250.13448736692577443No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC48980.13375007577714584TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105700.082.9661561
CGTAGGG10250.077.947592
GTAGGGC17550.072.3063053
AGTAGGG57200.071.893512
TAGGGCA16950.071.267014
TAGGGAC18100.071.153634
ACTAGGG18200.070.754952
ATAGGGA34850.070.397513
AAGGGAC36500.070.311264
AGTACGG3350.070.181451
GACCGAT2950.070.104178
AGGGACT58400.068.167585
TAAGGGA34900.067.872623
ACGGGAT9350.067.865224
TAGAGGG59000.067.469752
GGTAAGG21900.067.419061
GGGCGAT18700.067.359766
ATAGGGC19200.067.316423
AGGGATG52350.066.798025
TATAGGG28650.066.600742
Produced by FastQC (version 0.11.5)