FastQCFastQC Report
Mon 27 Feb 2023
SRR3127019.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127019.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927698
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239190.8169900037503869No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56680.19359920319650456No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG52550.17949255695088767No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG43110.14724879410376343No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC41330.1411689320414879TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80200.082.789941
CGTAGGG9000.079.595762
ACGGGAT6050.074.004324
TAGAGGG46400.071.0998842
GGTAAGG19450.071.039071
AAGGGAC28800.070.857244
AGTAGGG44950.070.772232
GTAGGGC14150.070.610333
TAGGGCA13750.070.265154
AGGGACT47250.069.3221365
AGTACGG3950.069.243681
ATAGGGC16150.068.5778963
AGAGGGC42100.068.286773
GACCGAT3100.068.216878
ACGGGTA2650.067.58134
TAAGGGA26500.067.58133
ATAGCGG5250.067.367621
ATAGGGA25450.067.221423
ATAGAGG18950.066.9410251
TAGGGAT19650.066.916084
Produced by FastQC (version 0.11.5)