FastQCFastQC Report
Mon 27 Feb 2023
SRR3127018.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127018.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3053455
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251720.8243776312406765No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT59930.19626947179506496No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG51910.1700041428480197No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG42540.13931759269417757No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC40090.13129389494850915TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81050.083.328771
ACGGGTA3400.074.642244
CGTAGGG8450.072.8997962
TAGGGCA14900.071.9148254
TAGAGGG53400.070.8869552
GTAGGGC15650.069.969933
ATAGGGC18000.069.712183
ATAGGGA27300.069.204333
GACCGAT2450.069.056778
TACGGGT1850.068.590173
AGGGTAC13650.068.515735
ATAGCGG6550.068.261911
ACGGGAT8550.068.159354
ATAGAGG20650.068.147091
GAATAGG19150.067.832281
AGTAGGG45200.067.725822
AGAGGGC45600.067.403553
AGGGCAT28900.067.324385
AGGGATG42450.067.31255
GGGCGAT14550.066.5386666
Produced by FastQC (version 0.11.5)