FastQCFastQC Report
Mon 27 Feb 2023
SRR3127016.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127016.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739340
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162750.9356997481803443No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG45340.26067358883254566No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41070.2361240470523302No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG38910.22370554348201044No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC35210.20243310681062932TruSeq Adapter, Index 16 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24170.1389607552289949No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54500.083.980941
ACGGGTA3650.082.61474
TAGGGCA9650.075.678874
CTACGAC8400.075.526545
ACGGGAT5500.075.385924
ATCGGTA255.4925535E-575.385913
AGGGATG34100.074.005685
GAATAGG11200.073.6508941
TACGACC8750.072.505486
CGTAGGG4950.072.34212
GCTACGA8800.072.2805254
TAGGGTC4050.072.1284944
TAGAGGG30300.071.9981162
AGGGCAT17300.071.713845
AGTAGGG26300.071.303372
AGGGAAT25950.070.808365
GGATACG605.456968E-1270.491448
CGCTACG9100.070.415413
ATAGGGA17650.070.207143
GGATGGC27150.070.101997
Produced by FastQC (version 0.11.5)