FastQCFastQC Report
Mon 27 Feb 2023
SRR3127014.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127014.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498803
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60431.2115003317943156No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG21000.4210078928955921No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC20730.4155949342726487No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG18390.36868262620713993No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9050.18143435384310036No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT7280.14594940287047192No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT5870.11768173006176787No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT5300.10625437296888751No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT5200.10424957347890851No Hit
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG5050.10124237424394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA700.087.2791064
CGTTTTT21850.085.199951
TAGGGCA1450.081.028354
CGAAGGG3800.079.151912
ATAGCGG600.078.350971
AGGGCAT3750.077.7007755
TAGCGGG2150.076.505842
AATAGGG5450.074.159522
AGTAAGG3000.073.649911
TAACGGG1150.073.559652
GGGAATG11400.072.5559166
GGTAAGG3050.072.442531
CGAGGGG4250.071.876913
AAGGGCG1900.071.7314154
AGGGCGA1900.071.7314155
TAGTAGG1450.071.326391
CATACGG200.002160330570.515871
GTTTGCG200.002162909470.494676
TACGGGT200.002162909470.494673
AACGGGT800.070.494673
Produced by FastQC (version 0.11.5)