FastQCFastQC Report
Mon 27 Feb 2023
SRR3127013.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127013.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405072
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44331.0943733459730616No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG19270.47571789706521306No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC19040.470039894142276No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG16610.41005055891298337No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6570.1621933878421614No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT6190.1528123395347No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT5450.13454398230438044No Hit
GATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC5040.1244223249200142No Hit
AGAGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT4780.11800371292017221No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT4300.10615396768969468No Hit
GAAGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT4270.10541335861278982No Hit
GGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT4180.10319153138207528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGG800.088.375132
CGTTTTT16800.086.184041
TAATAGG950.084.396231
TACGGGG2100.083.044573
GTTACGG401.0059011E-982.5345461
ACGGGTC401.0113581E-982.483464
TAGGGCG401.0113581E-982.483464
AACGGGC1100.081.412243
AGGGCTA1200.078.322945
AGTAAGG2200.077.175161
ACGGGTA551.8189894E-1277.127394
GCGGGTA800.076.591784
AGGGCAT2700.076.582435
AAGGGTA3150.076.3112264
AGGGATG3350.075.751155
TACGGGT255.47753E-575.4134453
CGGGTAA255.558686E-575.1900255
TAGTAGG1200.074.674121
TAGCGGG1200.074.627892
AGGGTAC1400.073.847345
Produced by FastQC (version 0.11.5)