FastQCFastQC Report
Mon 27 Feb 2023
SRR3127012.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127012.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418585
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44661.0669278641136208No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC20300.48496721096073675No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG18750.44793769485289725No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG15920.38032896544309996No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA7060.1686634733686109No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT6560.15671846817253365No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT5580.1333062579882222No Hit
GATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC5310.1268559551823405No Hit
GGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT4450.10631054624508762No Hit
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG4380.1046382455176368No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGG156.869123E-494.1030961
TACGGGT254.918602E-793.9906853
TAACGGG800.088.158392
AACGGGT500.084.591613
CGTTTTT18000.083.64721
CGAAGGG2700.083.587212
AGGGTAC1200.082.241855
GTAGGGT1550.081.862853
AAGGGTA2450.078.645264
TAGTAGG2050.078.036711
ATGTAGG1950.077.21281
AGGGCGA1650.076.901475
AGGGAAT8500.075.745445
CGGGTAT1000.075.192545
GCGATTG255.557979E-575.192548
CGATCAG255.557979E-575.192549
GCAAGGG5750.074.41082
AGTAAGG2550.073.806351
TAGATGG1150.073.6459051
TAGCGGG1100.072.663892
Produced by FastQC (version 0.11.5)