FastQCFastQC Report
Mon 27 Feb 2023
SRR3127011.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127011.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126006
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300740.9620582941939331No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT69150.22120878846681674No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC46290.1480803299801728No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG45870.14673676250141554No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG43280.13845142971574592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98050.083.589821
ATAGGGA28900.073.0191353
AGTAGGG45350.072.1272352
TAGGGCA17000.071.054764
AGGGATG45950.070.57745
ATAGGGC19000.069.508983
ATAGCGG5600.068.8540341
TAAGGGA28650.068.57093
GGTAAGG16900.068.446621
GTAGGGA25450.067.2205053
TAGGGAT22500.067.055064
AAGGGTA20950.066.856134
AGGGCAT29050.066.819925
TAGCGGG15350.066.744492
ATAAGGG45950.066.582922
CGTAGGG8900.066.5346452
AGGGAAT34250.066.280945
TAGACGG3850.065.9533161
AGAGGGC46750.065.748273
ATGAGGG47000.065.695382
Produced by FastQC (version 0.11.5)