FastQCFastQC Report
Mon 27 Feb 2023
SRR3127010.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127010.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482925
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218960.8818631251447386No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53000.21345791757705124No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG44180.1779352980859269No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC39020.15715335743125547No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG37330.1503468691160627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74850.083.722911
GTACGGG5800.075.56192
TAGGGCA12750.073.919494
AGGGCAT23600.071.8843845
TAGCGGG12750.070.964142
ATAAGGG34150.070.100472
AGGGATG37950.069.716555
AGTAGGG38000.068.950772
CGAAGGG22300.068.679392
ATAGGGA21550.068.225463
AGGGAAT29250.067.9600755
AAGGGTA15000.067.2297744
CGTAGGG6800.067.221892
AGAGGGC37150.067.102093
TAAGGGA22300.066.776153
ATAGGGC15800.066.211753
ATAGAGG16050.066.102611
ATAGCGG4500.066.014471
GGTAAGG13500.066.0144651
ATGAGGG31150.065.959342
Produced by FastQC (version 0.11.5)