FastQCFastQC Report
Mon 27 Feb 2023
SRR3127009.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127009.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647011
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231660.8751758115096613No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT59120.22334625734460492No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG43410.16399629619975134No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC39730.15009382280617647No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG37640.14219812460167336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79400.083.086381
TAGGGCA14000.072.845134
ATAGGGA24000.071.6702043
GGTAAGG16150.069.926381
TAAGGGA22800.069.464653
CGTAGGG8100.069.0865252
AGTAGGG38100.068.871672
ATAAGGG39950.068.860552
AGGGATG38550.068.636135
GTAGGGT10500.068.481133
ATAGAGG19200.068.131181
AATGCGG6850.068.005961
AGGGAAT29450.067.183965
AGGGCAT22050.066.71215
ATAGCGG5050.066.156011
GTACGGG5200.066.016262
AGAGGGC41150.066.0125963
ATGCGGG11800.065.755692
GAATAGG16500.065.59131
GTAGGGA23000.065.591263
Produced by FastQC (version 0.11.5)