FastQCFastQC Report
Mon 27 Feb 2023
SRR3127008.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127008.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3628591
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT525351.4478071515913478No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46640.12853473979293892No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42520.11718047032580965No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG37030.1020506306717952TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG36620.100920715506377TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT164800.086.597291
ACGGGAT9450.075.1090554
ATAGGGC22300.070.185053
AGTAGGG54800.070.06852
AGGGATG57100.070.05335
AGGGCAT32800.069.502925
TAGCGGG18000.069.452912
AGGGTAC15100.069.105285
ACGGGTA3850.068.37144
GAATAGG20650.068.085391
TAAGGGA36300.067.587923
AGGGAAT43100.067.39795
TAGAGGG62700.066.637012
ATAGGGA33750.066.149283
TAGGGCA20750.066.147274
ATAGCGG7350.065.895031
TAGGGTA12850.065.844054
AAGAGGG129250.065.561142
GACCGAT1650.065.5207758
AGGGATC27600.065.396925
Produced by FastQC (version 0.11.5)