FastQCFastQC Report
Mon 27 Feb 2023
SRR3127007.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127007.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885923
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391321.355961333687697No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34960.1211397532089387No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG34670.12013487539341831TruSeq Adapter, Index 13 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33690.1167390813961426No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG32470.11251166437912584TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC29860.1034677640394425TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118200.086.343361
AGTAGGG46050.072.64632
AGGGATG47250.071.610685
GTAGGGA24350.070.819333
CGTAGGG7400.070.039932
GAATAGG15750.069.737111
TAAGGGA24800.069.724283
TAGCGGG13800.069.652372
GGTAAGG15950.068.8626561
AGGGCAT24950.068.561135
TAGGGCA14650.067.86014
ATAGGGC16550.067.756143
CGAAGGG27550.067.7264562
TAGGGTA9100.067.308774
AAGAGGG98600.066.710362
TAGAGGG47650.066.5482942
ACGGGAT7050.066.163094
AGGGTAC12900.065.938035
ATAGGGA27950.065.743453
TACGGGT2300.065.552893
Produced by FastQC (version 0.11.5)