FastQCFastQC Report
Mon 27 Feb 2023
SRR3127006.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127006.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017962
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405121.3423628263046388No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37120.12299690983518016No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35670.11819234304474344No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG33870.11222805323592544TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG31440.10417626199402112TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126550.086.640891
AGGGATG49700.073.473745
CGTAGGG8500.073.014592
AGGGCAT26350.071.520625
ACGGGAT7950.069.7561344
AGTAGGG47150.068.605892
ATAGGGA29850.068.015353
ATAGGGC18250.067.98433
TAGGGCA14600.067.276124
TAAGGGA29150.067.069073
GAATAGG16900.066.827261
TAAGAGG22100.066.221231
AGGGACT37700.066.0696645
AGGGAAT35000.066.0639655
CGAAGGG27950.065.77332
AAGGGCG14600.065.666654
AGATAGG16650.065.569651
TAGCGGG15350.065.548092
AAGGGAC31150.065.0259554
GATAGGG45150.064.980262
Produced by FastQC (version 0.11.5)