FastQCFastQC Report
Mon 27 Feb 2023
SRR3127005.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127005.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3508470
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT952112.713747017930893No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT66290.18894275852437104No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT59450.16944708092131328No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT55470.15810310477216563No Hit
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT47780.13618471869504373No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG42660.12159146294538645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT291600.089.132431
AGGGATG59750.073.788755
TAGGGCA19900.071.8065954
ATAGGGC22600.071.541283
GGTAAGG18550.069.955271
ATAGGGA33000.069.219593
AGTAGGG51350.068.646962
ATAGCGG7550.067.87891
CGTAGGG9950.067.548152
AGATAGG20000.067.234371
TAGAGGG62200.067.100092
GTAGGGA24750.067.0357063
TAGGGAT24650.066.932034
AGGGAAT40950.066.802755
GAGGGAT48200.065.9239964
GTAGGGT13600.065.3175053
GATAGGG53550.064.861272
GTAGGGC17400.064.2886663
GTACGGG8200.064.195512
AGGGCAT31050.064.033035
Produced by FastQC (version 0.11.5)