FastQCFastQC Report
Mon 27 Feb 2023
SRR3127004.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127004.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2805624
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT702962.5055388747743814No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT63210.225297473930933No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT54550.19443090022041443No Hit
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT45280.16139012212612952No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44450.1584317784564147No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG36950.13169975734453368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT217500.089.4799351
AGGGATG44700.074.536725
ACGGGAT8150.074.044854
TAGGGCA13850.071.824934
ATAGGGC17100.071.6835863
ATAGGGA25000.070.90723
ATAGCGG4600.070.77051
GGTAAGG17700.068.504771
CGTAGGG8400.068.474812
TAAGGGA23250.067.727653
TAGGGAT17300.067.312134
GTAGGGC13750.067.204113
AGTAGGG42150.067.112582
TAGAGGG45750.066.5720142
GAGGGAT34500.066.4144
AGGGCAT23300.066.152995
ATAAGGG37000.066.0052262
AGTAAGG18050.065.869491
AGAGGGC41000.065.659073
GGATAGG16750.065.629951
Produced by FastQC (version 0.11.5)