FastQCFastQC Report
Mon 27 Feb 2023
SRR3127003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927327
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT735082.511096300481634No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT61050.2085520339886866No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT51760.1768165975307849No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46660.15939456029340077No Hit
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT46460.1587113431468367No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG35970.12287660380955048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT222750.089.244651
ATAGGGC18250.071.076883
AGGGATG48750.070.473355
AGATAGG15000.070.280171
ATAGGGA26100.070.227343
ACGGGAT8100.068.466664
AGTAGGG44350.068.274712
ATAAGGG36250.067.447052
TAGGGCA15600.065.677124
TAGAGGG51850.065.472082
AGTAAGG19900.065.2727661
GTAGGGC14200.065.2018663
CGTAGGG7650.065.149652
AATAGGG39950.065.084272
AGTACGG3400.065.057141
TAGGGAT19150.065.036814
GAGGGAT38900.064.87944
AGAGGGC45100.064.713853
AGGGAAT30700.064.603525
AAGGGCG13500.064.056944
Produced by FastQC (version 0.11.5)