FastQCFastQC Report
Mon 27 Feb 2023
SRR3127002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396890
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369381.0874064217563713No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41350.12172899328503424No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35090.10330037181068566No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT121550.083.324411
ACGGGTA5000.078.958534
ATAGCGG7450.074.474441
TAGGGCA18500.073.166194
ATAGGGC22200.073.037023
TAGGGTA10850.071.4733054
AGGGATG53500.070.36695
CGTAGGG9450.069.622182
AGGGCAT28650.069.2273255
TAGAGGG66100.068.3242
AGTAGGG50250.068.084252
AGATAGG22450.067.8594361
ATAGAGG27150.067.715551
CGAAGGG32600.067.609322
GGTAAGG17750.067.549691
ATGAGGG57050.067.053292
GTAGGGC14000.066.803923
AGAGGGC51050.066.745063
AAGAGGG120250.066.711362
ATAGGGA34850.066.349743
Produced by FastQC (version 0.11.5)