FastQCFastQC Report
Mon 27 Feb 2023
SRR3127001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2660356
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272591.024637304180343No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31980.12020947572430156No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG26970.10137740963991285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93150.083.512251
ACGGGTA3350.077.360874
TAGGGCA11600.075.1479644
ATAGCGG5300.073.833131
CGTAGGG7500.070.9938352
ATAGGGA26100.070.769973
AGGGATG40650.070.751195
AAGGGTA22350.069.572874
AGGGCAT21800.068.982095
GTAGGGC10800.068.934533
TAGAGGG50000.068.606442
ATAGGGC16750.068.358883
TATAGGG22100.067.5881352
TAGGGCG6350.067.5260244
AGTAGGG40950.067.429082
AGGGTAG16400.067.3390355
TAGGGTA10150.067.314014
GAATAGG17100.067.273311
AGAGGGC43200.066.862133
TAGGGAT19850.066.7036064
Produced by FastQC (version 0.11.5)