FastQCFastQC Report
Mon 27 Feb 2023
SRR3126999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670364
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198061.1857295775052623No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG41200.24665282537219432No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG38910.2329432387192253No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC32390.19390983043216928TruSeq Adapter, Index 22 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25130.15044625003891368No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17980.10764120874252557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60650.084.66821
CGTAGGG4050.077.7501452
ATAGCGG2900.076.212871
TAGTAGG6800.073.303621
AGAGGGC20750.072.7078553
ATAGGGC8450.071.750913
GAATAGG9900.070.774951
TAGGGTA6000.070.499444
ACGGGAT3400.070.4994354
AGATAGG8200.069.3904951
AACGAGG7050.069.370161
AGTAGGG23900.069.219132
AGGGCAT12500.069.181375
AAGGGAC16200.069.0488364
AAGAGGG53200.067.758712
ATAGAGG10300.067.5698551
ATAGGGA14550.067.511493
AGGGAAT19450.066.93325
GGTAAGG7750.066.745111
TAGAGGG27050.066.718362
Produced by FastQC (version 0.11.5)