FastQCFastQC Report
Mon 27 Feb 2023
SRR3126998.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126998.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327276
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142591.0743055702054434No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG38880.2929307845542299No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG34510.2600062082038702No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC28540.2150268670570401TruSeq Adapter, Index 22 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19520.14706813051693846No Hit
AGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT15320.11542437292620374No Hit
AGAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT13390.10088331289046137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46300.085.592741
TAGTAGG4750.077.4710851
CGTAGGG2950.076.723422
AGATAGG7200.076.008851
ATAGGGC7150.075.172383
GAATAGG7650.074.621261
TAGAGGG21150.073.3490142
AGTAGGG18750.072.426922
TAGGGTA4150.071.5734564
GGTAAGG7150.070.60191
TAACGGG2750.070.300742
ATAGAGG8600.070.218281
GGATAGG6350.069.8381651
AGGGCAT8650.069.538885
GTAGGGA7650.069.026543
ACGAGGG10800.068.54642
AGGGTAC5500.068.353555
GTAGGGC5450.068.3423843
CGGGTAT2000.068.1399465
TAGGGCA6200.067.679614
Produced by FastQC (version 0.11.5)