FastQCFastQC Report
Mon 27 Feb 2023
SRR3126997.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126997.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393458
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146421.0507672280040017No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG38350.2752146099846569No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG33210.23832795821617875No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC28280.2029483486405762TruSeq Adapter, Index 22 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20790.149197177094681No Hit
AGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT14850.10656941221048642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT45600.083.794731
GAATAGG7050.081.432221
CGTAGGG2950.076.504332
ATAGGGC6750.074.50063
ACGGGAT2600.072.304694
GTAGGGA9500.072.228583
AATAGGG16400.071.100832
AAGGGCG5250.070.720874
GGTAAGG6400.070.585721
GGATAGG7200.070.585711
TAGGGAC6200.070.497074
ATAGGGA11100.069.861953
TATAGGG9500.069.785052
TAGGGCA5800.069.686754
AGTAGGG19100.069.17352
TAGTAGG5050.068.02321
ATAGGGT5050.067.9377753
TACGAGG2150.067.849831
ACGAGGG12150.067.334842
AGGGCAT10200.067.271715
Produced by FastQC (version 0.11.5)