FastQCFastQC Report
Mon 27 Feb 2023
SRR3126996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904263
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140081.5491068417042386No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG37990.4201211373239865Illumina PCR Primer Index 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG34030.3763285681267507Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC30130.3331995227052307Illumina PCR Primer Index 2 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14390.15913511887581377No Hit
AGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT13050.1443164212181633Illumina PCR Primer Index 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT10190.11268845457571525Illumina PCR Primer Index 2 (95% over 22bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.10129796309259584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT39550.087.27261
GTAGGGC2800.085.6052253
TAGACGG1000.084.644911
CGTAGGG1950.081.9468842
GTAGGGT3750.077.7049263
GTACGGG1400.077.2125552
GGTAAGG4150.077.052931
AGGGAAT13250.076.976115
AGTAGGG12300.076.421062
GACCGAT800.076.377528
TAGGGTC2100.076.101954
TACGGGT255.559352E-575.1983263
ACGGGAT2200.074.779334
GTAGGGA6850.073.414423
TAGGGCA3750.072.6997454
CGAAGGG8050.072.39592
AGGGCTC6550.071.758095
AGAGGGC10850.070.606713
TGTAGGG5750.070.2940752
TAGTAGG2950.070.1389161