Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126996.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 904263 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 14008 | 1.5491068417042386 | No Hit |
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG | 3799 | 0.4201211373239865 | Illumina PCR Primer Index 2 (95% over 23bp) |
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG | 3403 | 0.3763285681267507 | Illumina PCR Primer Index 2 (95% over 23bp) |
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC | 3013 | 0.3331995227052307 | Illumina PCR Primer Index 2 (95% over 24bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 1439 | 0.15913511887581377 | No Hit |
AGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT | 1305 | 0.1443164212181633 | Illumina PCR Primer Index 2 (95% over 22bp) |
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT | 1019 | 0.11268845457571525 | Illumina PCR Primer Index 2 (95% over 22bp) |
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 916 | 0.10129796309259584 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 3955 | 0.0 | 87.2726 | 1 |
GTAGGGC | 280 | 0.0 | 85.605225 | 3 |
TAGACGG | 100 | 0.0 | 84.64491 | 1 |
CGTAGGG | 195 | 0.0 | 81.946884 | 2 |
GTAGGGT | 375 | 0.0 | 77.704926 | 3 |
GTACGGG | 140 | 0.0 | 77.212555 | 2 |
GGTAAGG | 415 | 0.0 | 77.05293 | 1 |
AGGGAAT | 1325 | 0.0 | 76.97611 | 5 |
AGTAGGG | 1230 | 0.0 | 76.42106 | 2 |
GACCGAT | 80 | 0.0 | 76.37752 | 8 |
TAGGGTC | 210 | 0.0 | 76.10195 | 4 |
TACGGGT | 25 | 5.559352E-5 | 75.198326 | 3 |
ACGGGAT | 220 | 0.0 | 74.77933 | 4 |
GTAGGGA | 685 | 0.0 | 73.41442 | 3 |
TAGGGCA | 375 | 0.0 | 72.699745 | 4 |
CGAAGGG | 805 | 0.0 | 72.3959 | 2 |
AGGGCTC | 655 | 0.0 | 71.75809 | 5 |
AGAGGGC | 1085 | 0.0 | 70.60671 | 3 |
TGTAGGG | 575 | 0.0 | 70.294075 | 2 |
TAGTAGG | 295 | 0.0 | 70.138916 | 1 |