FastQCFastQC Report
Mon 27 Feb 2023
SRR3126995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748174
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99301.327231365965671No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG35610.47595880102756843Illumina PCR Primer Index 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG30210.40378307719861956Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC25500.34082980697003634Illumina PCR Primer Index 2 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT12030.16079147364115834Illumina PCR Primer Index 2 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT11170.14929682132765906No Hit
GGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT10230.13673289903150873Illumina PCR Primer Index 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT9740.13018362038777076Illumina PCR Primer Index 2 (95% over 22bp)
GGAGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT8490.1134762769088474No Hit
AGAGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT7670.10251625958667368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT27650.087.6696851
ACGGGAT1300.087.013624
TAGACGG1300.083.4383851
GGTAAGG3800.081.910921
GTAGGGT2750.080.553523
TAGGGTC1350.080.2996144
GTACGGG1250.079.187712
TAGGGTA2400.078.553974
CGTAGGG1350.076.813482
AGGGATG5850.075.5113755
AGTAGGG9200.073.265022
GGGCGAT2000.072.840376
TAGTAGG1750.072.762421
ATGAGGG9800.072.627222
AGGGAAT12200.072.031455
AACGAGG2050.071.3163761
ATAGGGC3400.070.698573
TACGGGT200.002138690370.698573
GCGAGGG5400.069.830442
AGGGCTC5200.069.5869455
Produced by FastQC (version 0.11.5)