FastQCFastQC Report
Mon 27 Feb 2023
SRR3126994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769616
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105101.3656160994573916No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG34250.4450271304130891Illumina PCR Primer Index 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG27710.360049687116692Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC24220.3147023970395626Illumina PCR Primer Index 2 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT12010.15605184923390367No Hit
AGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT11710.15215380137627077Illumina PCR Primer Index 2 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT10350.13448265108833496Illumina PCR Primer Index 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT9460.12291844244402403Illumina PCR Primer Index 2 (95% over 22bp)
GGAGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT8370.10875553522795783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT29550.086.957561
ACGGGAT1350.083.551984
TAGGGTC1550.081.867464
GATTAGG2100.080.6780551
GTAGGGT2000.079.8965763
TTTACGG301.4442794E-678.4371
ACGGATA301.4560537E-678.329984
TAGGGCA3050.077.0458764
ACTAGGG2400.076.4164052
TAGGGTA1550.075.803214
GCGTTAT255.5593922E-575.196789
AGGGAAT10450.075.106835
CGTAGGG1200.074.457012
AGTAGGG9050.074.305462
AGGGCTC4700.073.9968265
GGGCAAT1350.073.107986
AGTAAGG4800.072.554221
AGACGGG4150.072.5212252
ATAGAGG4100.072.315081
ATAGGGC3400.071.879273
Produced by FastQC (version 0.11.5)