FastQCFastQC Report
Mon 27 Feb 2023
SRR3126993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3106534
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311771.0035943595016183No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT84680.2725867478031787No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG45310.14585386800852654No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG39130.1259603146142936No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC38430.12370699950491448TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96150.082.9788361
ACGGGAT8850.073.81994
ATAGGGC20650.073.739293
TAGGGCA16750.071.272314
AGTAGGG50950.070.934272
AGGGATG45650.069.7016145
ATAGCGG6300.067.910291
GGTAAGG18400.067.711561
AGGGCAT25000.067.115555
CGAAGGG33750.066.7015152
AAGAGGG107800.066.6160052
TAGAGGG56450.066.2709352
ATAGGGA26200.066.191023
AGAGGGC43400.066.056283
AGTAAGG18950.065.994421
GAATAGG16750.065.961091
AGGGACT56700.065.7332465
TAAGGGA27400.065.521943
TAGGGCG8250.065.515774
GAGGGAT35400.065.322654
Produced by FastQC (version 0.11.5)