FastQCFastQC Report
Mon 27 Feb 2023
SRR3126992.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126992.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442025
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227190.9303344560354624No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT66980.27428056633326847No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG40410.1654774214023198No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG35230.1442655173472835No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC32720.1339871622935883TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69000.084.812081
ATAGGGA18850.073.510263
ACGGGAT6550.073.3957444
AGTAGGG37500.071.391562
GGTAAGG14900.070.891921
AGGGATG34750.069.915635
TAGGGCA12600.069.575194
ATAGGGC14800.069.423543
GAATAGG13150.068.8510361
ATAGCGG5700.068.665451
TAGGGCG5150.067.723054
AAGAGGG81650.067.597492
AGAGGGC36650.067.0001453
CGTAGGG6300.066.585452
TATAGGG17700.066.572762
TAGAGGG43450.066.4967652
CGAAGGG23800.066.145352
AGGGCAT20300.065.976215
TAAGGGA20200.065.5642243
AGTAAGG15250.065.5542761
Produced by FastQC (version 0.11.5)