FastQCFastQC Report
Mon 27 Feb 2023
SRR3126991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2580424
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235990.9145396260459522No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT72170.2796827188090019No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG38840.1505178993839772No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG34310.13296264489866783No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC33360.12928107938850358TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71250.084.473611
ATAGGGC17200.075.4154363
AGGGATG35400.070.497045
TAGGGCA14550.070.093284
GGTAAGG15250.070.0472951
AGGGCAT21950.069.372945
ATAGCGG5650.069.129751
ACGGGAT7100.068.842174
ATAGGGA20400.068.653983
GAATAGG13300.068.287471
TAGGGCG6150.068.0134054
AGTAGGG43350.067.483992
TAGAGGG46750.067.405092
GAGGGAT27550.067.213144
AGGGACT46150.067.110945
AAGAGGG87300.067.020192
TAGCGGG13700.066.944292
GGATAGG15600.066.665791
AATAGGG36350.065.470492
CGAAGGG26800.065.1086352
Produced by FastQC (version 0.11.5)