FastQCFastQC Report
Mon 27 Feb 2023
SRR3126990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058867
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123171.1632244653955597No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG36330.34310258039961583TruSeq Adapter, Index 13 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31490.29739334590652083No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG30620.28917701656581984TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC27820.2627336577681616TruSeq Adapter, Index 8 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT12590.11890067402232764TruSeq Adapter, Index 8 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT12370.11682298154536877No Hit
GGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT10600.10010700116256338TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT42850.085.697091
CGGGTAT2050.084.8260355
TCCGCTA5850.082.784161
CGTAGGG2450.080.568352
ACGGGTA1600.079.309474
AGTAGCG301.456392E-678.3303459
GTACGGG1800.078.3303452
TAGGGCA6150.077.1840444
AGGGATG21050.076.804675
CGCTACG6300.076.092333
CTACGAC6350.075.493195
CGGGTAA1000.075.197135
GGTAAGG6150.074.923291
GTAGGGA8650.073.8931353
GGGCGAT3400.073.261916
GACCGAT900.073.108328
AGGGCAT10250.072.904535
AGGGTAT9350.072.884925
TAGACGG1550.072.802361
TACGACC6700.072.250986