FastQCFastQC Report
Mon 27 Feb 2023
SRR3126989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862945
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89311.0349442896128955No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG34330.3978237315240253TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG28340.3284102694841502TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC25770.2986285336840702TruSeq Adapter, Index 8 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24180.28020325744977953No Hit
AAAGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT12590.14589574074825162No Hit
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT11810.13685692599180713TruSeq Adapter, Index 8 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT11620.1346551634229296TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT10500.12167635249059905TruSeq Adapter, Index 8 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT9520.1103198929248098No Hit
GGAGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT9440.1093928350010719No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT31000.085.777631
TAGCGGG4250.079.8312762
CGCTACG4500.079.584883
CGTAGGG1600.079.519432
ACGGGTA1150.077.8547744
GCGTAGG850.077.654341
TCCGCTA4900.076.0129551
TACGTAA255.561939E-575.1908659
AAGGGTC4400.074.967814
CGGGTAT1950.074.708875
AAGGGTA7400.074.504694
AGGGATG14900.074.4339145
GTACGGG1900.074.4041442
GGTAAGG4950.074.292691
TAGGGCA4700.074.193074
GTAGGGA6700.073.848893
TAGACGG1150.073.7957461
AGGGCAT6650.073.4948355
ATAGCGG1800.073.340221
TAGGGAC4050.073.3018654
Produced by FastQC (version 0.11.5)