Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126988.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 888230 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 9320 | 1.0492777771523143 | No Hit |
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG | 3266 | 0.3676975558132466 | TruSeq Adapter, Index 13 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG | 2746 | 0.30915416052148653 | TruSeq Adapter, Index 13 (95% over 22bp) |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 2538 | 0.2857368024047825 | No Hit |
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC | 2518 | 0.28348513335509945 | TruSeq Adapter, Index 8 (95% over 23bp) |
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT | 1175 | 0.1322855566688808 | TruSeq Adapter, Index 8 (95% over 21bp) |
AAAGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT | 1152 | 0.12969613726174525 | No Hit |
GGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT | 1104 | 0.12429213154250589 | TruSeq Adapter, Index 13 (95% over 21bp) |
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT | 989 | 0.11134503450682819 | TruSeq Adapter, Index 8 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 3020 | 0.0 | 86.93202 | 1 |
TAGGGCG | 145 | 0.0 | 84.26916 | 4 |
ACGGGTA | 140 | 0.0 | 80.565 | 4 |
TAGACGG | 165 | 0.0 | 79.84112 | 1 |
AGGGATG | 1720 | 0.0 | 78.96464 | 5 |
AGGGCAT | 850 | 0.0 | 77.4056 | 5 |
TAGGGTC | 180 | 0.0 | 75.71619 | 4 |
TAAGGGA | 800 | 0.0 | 74.60656 | 3 |
CGCTACG | 505 | 0.0 | 73.51889 | 3 |
AGACGGG | 570 | 0.0 | 73.40491 | 2 |
ACGCGGG | 180 | 0.0 | 73.12998 | 2 |
GACGGGT | 135 | 0.0 | 73.105286 | 3 |
AGAGGGT | 715 | 0.0 | 72.95923 | 3 |
GTAAGGG | 1275 | 0.0 | 72.63836 | 2 |
GGTAAGG | 545 | 0.0 | 72.516235 | 1 |
GCGGGAT | 260 | 0.0 | 72.301926 | 4 |
GTAAAGG | 430 | 0.0 | 72.215096 | 1 |
TCCGCTA | 515 | 0.0 | 72.1726 | 1 |
AGCGGGT | 275 | 0.0 | 71.7761 | 3 |
AGGGAAT | 1450 | 0.0 | 71.62878 | 5 |