FastQCFastQC Report
Mon 27 Feb 2023
SRR3126988.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126988.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888230
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93201.0492777771523143No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG32660.3676975558132466TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG27460.30915416052148653TruSeq Adapter, Index 13 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25380.2857368024047825No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC25180.28348513335509945TruSeq Adapter, Index 8 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT11750.1322855566688808TruSeq Adapter, Index 8 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT11520.12969613726174525No Hit
GGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT11040.12429213154250589TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT9890.11134503450682819TruSeq Adapter, Index 8 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT30200.086.932021
TAGGGCG1450.084.269164
ACGGGTA1400.080.5654
TAGACGG1650.079.841121
AGGGATG17200.078.964645
AGGGCAT8500.077.40565
TAGGGTC1800.075.716194
TAAGGGA8000.074.606563
CGCTACG5050.073.518893
AGACGGG5700.073.404912
ACGCGGG1800.073.129982
GACGGGT1350.073.1052863
AGAGGGT7150.072.959233
GTAAGGG12750.072.638362
GGTAAGG5450.072.5162351
GCGGGAT2600.072.3019264
GTAAAGG4300.072.2150961
TCCGCTA5150.072.17261
AGCGGGT2750.071.77613
AGGGAAT14500.071.628785