FastQCFastQC Report
Mon 27 Feb 2023
SRR3126987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3787051
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT466031.2305881278070985No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56430.14900776356061748No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47340.12500491807477637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT138050.085.599931
TAGGGCA22400.072.5974964
AGTAGGG60550.072.184712
ATAGGGC25250.071.660773
AGGGATG58000.071.2257845
AGGGAAT38450.070.893775
ATAGGGA36400.070.6265643
GGTAAGG22700.069.189131
AGTAAGG28750.067.877761
CGTAGGG13150.067.5482
GACCGAT2950.066.911948
ATAGCGG7050.066.7003561
TAGAGGG70900.066.552422
GAGGGAT43450.066.091414
ACGGGAT10500.065.7993554
GATAGGG56850.065.639682
AATAGGG55750.065.333092
AAGAGGG128650.065.2816542
TAGGGAT26650.064.8999254
AGGGCAT33750.064.89165
Produced by FastQC (version 0.11.5)