FastQCFastQC Report
Mon 27 Feb 2023
SRR3126986.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126986.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2974795
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343331.1541299484502294No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43440.14602686907837348No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG34380.11557098892528729No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34190.1149322894518782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105300.085.4370651
ATAGGGC19200.076.334563
AGGGAAT30400.071.263735
CGTAGGG7100.071.02232
TAGGGCA15450.070.765494
AGGGATG44900.070.543145
GGTAAGG18550.070.1554641
AGTAGGG46150.069.643722
ATAGGGA25250.069.056153
TAGCGGG14800.068.143022
TAGAGGG52450.067.657952
ATAGAGG22200.067.5414661
TAAGGGA23000.067.00113
AGAGGGC41450.066.7383653
TAGACGG4900.066.397131
ACGGGTA4050.066.32574
ACGGGAT8700.066.084954
GATAGGG41850.065.763762
GTAGGGC15050.065.757423
GCATAGG8750.065.742781
Produced by FastQC (version 0.11.5)