FastQCFastQC Report
Mon 27 Feb 2023
SRR3126985.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126985.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3092518
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357241.1551751679375837No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47160.15249709136697023No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35400.11446982685306925No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG34020.11000744377235637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107150.085.4207151
ATAGGGC19100.075.294243
ATAGCGG6350.074.848551
ACGGGAT9850.074.43244
ACGGGTA3600.073.107034
ATAGGGA30550.072.1498263
AGGGATG46750.071.576765
TAGGGCA17050.071.116274
AGTAGGG46950.069.095332
GATAGGG45550.068.845042
GAGGGAT32650.068.085034
GGTAAGG20550.068.011191
TAAGGGA26450.066.986823
CTACGAC8000.066.971275
GTAGGGC16000.066.3838043
ATAGAGG21100.066.238391
TAGGGAT21400.066.103794
AGGGAAT33900.065.990425
CGTAGGG9150.065.7694242
ATGCGGG15300.065.759352
Produced by FastQC (version 0.11.5)