FastQCFastQC Report
Mon 27 Feb 2023
SRR3126984.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126984.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3129760
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT846412.7043926690864475No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT72050.23020934512550484No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT61210.19557410152855173No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT49810.15914958335463422No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG44520.14224732886866726No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36060.11521650222381269No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC33740.10780379326210317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT243300.088.901821
GACCGAT2400.074.424278
ATAGGGC19400.071.955213
AGGGATG43050.069.4426355
TATAGGG23650.068.5638962
GTAGGGA24050.068.400693
GTAGGGC14300.068.365163
AGTAGGG49750.068.210562
AGATAGG16400.067.378121
AGGGCAT29300.066.897615
AGTAAGG22650.066.84711
ACGGGAT7750.066.719694
AGAGGGC48150.066.572613
ATAGGGA24600.066.489283
TAGGGCG4850.065.90684
TAGCGGG16550.065.318672
GAGGGAT33950.065.0760354
TAGAGGG51950.064.960062
AGGGAAT37050.064.5760965
TAGGGCA16800.064.35494
Produced by FastQC (version 0.11.5)