FastQCFastQC Report
Mon 27 Feb 2023
SRR3126983.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126983.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460820
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT626842.547280987638267No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT65690.26694353914548813No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT55000.2235027348607375No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT49140.19968953438284798No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG37100.15076275387878837No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28260.1148397688575353No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC27660.11240155720450906No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC25560.10386781641891728No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG25190.10236425256621776No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC25100.10199852081826384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT181500.089.210371
AGGGATG32800.072.066965
ATAGGGC14650.071.09853
AGTAGGG38050.070.6049042
TAGGGCG4350.069.3420264
TAGAGGG39500.069.32562
AGAGGGC37400.069.1841353
ATAGAGG15950.068.287781
AGGGAAT28100.067.898755
ATAGGGA21600.067.859773
TAGGGCA12650.067.80894
TAGCGGG13000.067.797382
GAGGGAT25950.067.7454
AGATAGG12300.067.4682161
GTAGGGC11600.067.445953
ACGGGAT5800.066.633354
AGGGTAC11950.066.460095
GAATAGG14050.066.447791
CGTAGGG5200.066.165892
AAGGGTA17700.066.036524