FastQCFastQC Report
Mon 27 Feb 2023
SRR3126981.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126981.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3075530
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261470.8501624110315945No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56010.18211495254476462No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG35620.11581743634430487No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG32150.10453482814344194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91050.083.0447851
ACGGGAT7150.073.631374
ATAGGGA25350.071.1970143
AGTAGGG50200.071.061192
ATAGGGC21300.070.612063
TAGGGCA17600.070.241534
AGGGATG45100.069.9308245
GAATAGG19800.069.821381
ATAGAGG19150.068.5080641
AAGAGGG111850.067.652572
TAGAGGG52350.067.4244842
TAGGGAT19600.066.671414
AGTAAGG21700.066.308311
AGGGACT45600.066.27795
AGGGTAC13750.065.974675
ATGCGGG17100.065.9643862
ATGAGGG52400.065.835352
ATAAGGG44400.065.5242542
CGTAGGG7700.065.3111652
GTAGGGC14900.065.2969063
Produced by FastQC (version 0.11.5)