FastQCFastQC Report
Mon 27 Feb 2023
SRR3126980.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126980.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447242
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195250.7978369119196221No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42730.17460471829103946No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG31810.129983058479709No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG29320.11980833934690562No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC26550.10848947509073478TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72150.082.869811
TAGGGCA13800.075.476724
ATAGGGC16450.074.778113
GTACGGG5400.072.4409562
AGGGATG34300.072.339885
AGTAGGG39400.070.216922
ACGGGAT5200.069.789044
ATAGGGA21150.069.748333
GGTAAGG15250.069.261461
GAGGGAT33150.068.953784
AAGAGGG88600.068.088872
AGGGCAT23700.067.416875
TAGGGAT14000.067.328944
GAATAGG14050.067.122351
TAGAGGG40900.066.835082
AGTACGG3350.066.155591
ATGCGGG14200.065.716842
TATAGGG17500.065.443732
ATGAGGG42450.065.393932
GATAGGG38250.065.058242
Produced by FastQC (version 0.11.5)