FastQCFastQC Report
Mon 27 Feb 2023
SRR3126979.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126979.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2565271
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204830.798473143773114No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45910.17896744632438444No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG30850.12026019863008626No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG27320.10649946925685434No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70800.083.549351
ATAGGGC15900.072.712913
ACGGGAT5300.072.7129064
TAGGGCA11700.071.9019554
AGTAGGG40800.071.4468152
GAATAGG13100.070.7678451
AGGGATG36100.069.12915
ATAGGGA20900.068.584673
ATAGAGG16400.068.579631
TAGGGTC8100.068.46534
TAGAGGG45550.067.402532
GAGGGAT33200.066.815544
GGTAAGG15800.066.418471
AGTAAGG15800.066.120631
GATAGGG39600.066.013232
GTAGGGC10600.065.6189653
AAGAGGG91250.065.591392
AAGGGAC22650.065.568074
ATAAGGG39250.065.284222
CGAAGGG24250.065.144672
Produced by FastQC (version 0.11.5)